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Enzyme calculation

john.matheson · 2017-01-11 13:56:49

Hi All, Quick question - If I have an enzyme extract of unkown activity. And I have the corresponding colorimteric substrate. How do I calculate activity of the enzyme extraction in U/ml, if 1 unit is the amount of activity which hydrolyses 1 umol sub per min Many thanks,

11-01-2017 1:56pm

#1

john.matheson

Registered Users, Registered Users 2 Posts: 1 ✭

Join Date: January 2017

Posts: 0

Hi All,

Quick question - If I have an enzyme extract of unkown activity. And I have the corresponding colorimteric substrate. How do I calculate activity of the enzyme extraction in U/ml, if 1 unit is the amount of activity which hydrolyses 1 umol sub per min

Many thanks,

0