About protein "denaturation" in water

maxi-twist

Does anyone have any proper info on this?Links etc..
I hear often on boards that your shakes become useless after being left in water for 20 mins.
But from my limited lc biology knowledge i understand that it has nothing to do with denaturation as that happens to enzymes and whatnot.

Even if the protein was broken down into amino acids in the shake after 20 mins,why would that matter as the exact same thing happens in your stomach?

Im just curious!

Degsy

I wouldnt imagine the act of denaturing would affect its bioavailability.After all the protein isnt being changed into a different compound and it still contains the same amount of amino acids.Protein like eggs "denature" at high temperature but it doesnt affect the nutritional quality of the food.

g'em

Your LC Biology is indeed limited - enzymes are proteins, and denaturation is a process common to all proteins. All proteins rely on their shape to be able to do what they do (enzymes have a certain shape to fit to their substrate, antibodies have a shape to bind to antigens, collagen has a shape to make it strucutrally sound) and denaturation changes that shape. Heating and cooling, the addition of chemicals and exposure to UV light among other things all contribute towards denaturing a protein (one reason why runninga high temperature for a long time is so dangerous is because our enzymes begin to denature and won't function).

When whey protein is manufactured the whey gets isolated and purified. The amino acids that make up the proteins can get structurally weakened via this process. They whey protein we buy is therefore very pure, but often not particularly stable. The addition of water hydrolyses the peptide bonds between the amino acids and can break apart the amino acids themselves. The proteins are getting "denatured". So the sooner you drink a whey shake after hydration, the more protein you will be able to absorb.

Degsy

g'em wrote:

Your LC Biology is indeed limited - enzymes are proteins, and denaturation is a process common to all proteins. All proteins rely on their shape to be able to do what they do (enzymes have a certain shape to fit to their substrate, antibodies have a shape to bind to antigens, collagen has a shape to make it strucutrally sound) and denaturation changes that shape. Heating and cooling, the addition of chemicals and exposure to UV light among other things all contribute towards denaturing a protein (one reason why runninga high temperature for a long time is so dangerous is because our enzymes begin to denature and won't function).

When whey protein is manufactured the whey gets isolated and purified. The amino acids that make up the proteins can get structurally weakened via this process. They whey protein we buy is therefore very pure, but often not particularly stable. The addition of water hydrolyses the peptide bonds between the amino acids and can break apart the amino acids themselves. The proteins are getting "denatured". So the sooner you drink a whey shake after hydration, the more protein you will be able to absorb.

#


Thanks for the lesson.So this is going to make HOW big a difference to anybody looking to supplement with whey?

g'em

Degsy wrote:

#


Thanks for the lesson.So this is going to make HOW big a difference to anybody looking to supplement with whey?

none. but the OP asked a question and I answered it. Wheres the problem? Are you bitter coz you got beat by a gurrl?

Degsy

g'em wrote:

none. but the OP asked a question and I answered it. Wheres the problem? Are you bitter coz you got beat by a gurrl?

If you knew me at all you'd know i'd quite LIKE to get "beaten" by a gurrl!;)

Vegeta

please some of us are trying to eat

Now I cant get the image of degsy in an assless gimp suit being spanked with a leather bound paddle by g'em out of my mind. :eek:

Degsy

Vegeta wrote:

please some of us are trying to eat

Now I cant get the image of degsy in an assless gimp suit being spanked with a leather bound paddle by g'em out of my mind. :eek:

You're one sick puppy,vegata.:D

cowzerp

the biggest problem is glutamine which is probably the most important amino acid of all is destroyed after a short period of time in water...

maxi-twist

g'em wrote:

Your LC Biology is indeed limited - enzymes are proteins, and denaturation is a process common to all proteins. All proteins rely on their shape to be able to do what they do (enzymes have a certain shape to fit to their substrate, antibodies have a shape to bind to antigens, collagen has a shape to make it strucutrally sound) and denaturation changes that shape. Heating and cooling, the addition of chemicals and exposure to UV light among other things all contribute towards denaturing a protein (one reason why runninga high temperature for a long time is so dangerous is because our enzymes begin to denature and won't function).

When whey protein is manufactured the whey gets isolated and purified. The amino acids that make up the proteins can get structurally weakened via this process. They whey protein we buy is therefore very pure, but often not particularly stable. The addition of water hydrolyses the peptide bonds between the amino acids and can break apart the amino acids themselves. The proteins are getting "denatured". So the sooner you drink a whey shake after hydration, the more protein you will be able to absorb.

But of course,so simple!
Cheers!

hardtrainer

See new thread. There is simple biology behind all of this, but nobody here seems to know it!

g'em

hardtrainer wrote:

See new thread. There is simple biology behind all of this, but nobody here seems to know it!

Gosh thanks for that. I wish I had the time to write lengthy posts pointing out other people's mistakes. Yeah, I know, I shouldn't make them in the first place... oh wait, I'm human. Sometimes it comes with the territory.

Dragan

hardtrainer wrote:

See new thread. There is simple biology behind all of this, but nobody here seems to know it!

Jesus Christ mate, chillax. People make mistakes. Even people who post here. I know, it's ****ing crazy isn't it?

Degsy

g'em wrote:

none. but the OP asked a question and I answered it. Wheres the problem? Are you bitter coz you got beat by a gurrl?

Yeah,chillax!

g'em

Degsy wrote:

Yeah,chillax!

:rolleyes: cheeky baxstard