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Cq and Ct in qPCR?

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  • 12-08-2010 10:28am
    #1
    IrishKnight
    Registered Users Posts: 922 ✭✭✭


    Can anyone explain what the differeance is between quantification cycle value (Cq) and the cycle threshold value (Ct)?

    I have an understanding that the Ct value is the vaule at which the fluorescence from a sample crosses a threshold but I haven't come across Cq before...


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    #2
    Rubecula
    Registered Users Posts: 8,551 ✭✭✭Rubecula


    I am not so sure on this myself, so I stand to be corrected.

    Ct is the threshold value.
    Cq is the absolute value. (it may be higher than the Ct)


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    #3
    IrishKnight
    Registered Users Posts: 922 ✭✭✭IrishKnight


    Hmm, from reading a bit more and a few more Google searches, it would appear that they are the same thing, just two different ways of saying the same thing...


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    #4
    adamski8
    Closed Accounts Posts: 4,503 ✭✭✭adamski8


    IrishKnight wrote: »
    Hmm, from reading a bit more and a few more Google searches, it would appear that they are the same thing, just two different ways of saying the same thing...
    im pretty sure thats right, think there is a newer name for it (cant remember which one) as there was confusion over the other one being used


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    #5
    jendafer1
    Registered Users Posts: 151 ✭✭jendafer1


    Is the Cq perhaps the same as the Rq? In which case Rq refers to the fold change vs one of your samples (usually the first?)


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    #6
    IrishKnight
    Registered Users Posts: 922 ✭✭✭IrishKnight


    jendafer1 wrote: »
    Is the Cq perhaps the same as the Rq? In which case Rq refers to the fold change vs one of your samples (usually the first?)

    Nope, turns out that Cq=Ct just a different way of saying one thing


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