Advertisement
If you have a new account but are having problems posting or verifying your account, please email us on hello@boards.ie for help. Thanks :)
Hello all! Please ensure that you are posting a new thread or question in the appropriate forum. The Feedback forum is overwhelmed with questions that are having to be moved elsewhere. If you need help to verify your account contact hello@boards.ie
Hi there,
There is an issue with role permissions that is being worked on at the moment.
If you are having trouble with access or permissions on regional forums please post here to get access: https://www.boards.ie/discussion/2058365403/you-do-not-have-permission-for-that#latest

Calibration curve

  • 28-04-2016 12:44pm
    #1
    okman
    Registered Users, Registered Users 2 Posts: 17 ✭


    Hi All - I posted this also in the chemistry and physics form. I hope this isnt breaking any rules just its something biologists might be able to help with also.

    I recently did some experiments where i had to use a p-nitrophenol to create a calibration curve in order to calculate concentrations of p-nitrophenol in an assay.

    Now I realise these calibration curves are likely a little out, and i am potentially over estimating the concentrations of p-nitrophenol in my assay.

    Is it possible however, to use the extinction coefficients found in the literature for p-nitrophenl and calculate my concentrations using this data as opposed to that of my own calibration curves?

    Cheers


Comments

  • #2
    Capt'n Midnight
    Moderators, Recreation & Hobbies Moderators, Science, Health & Environment Moderators, Technology & Internet Moderators Posts: 93,563 Mod ✭✭✭✭Capt'n Midnight


    The whole point of a calibration curve is to take into account local variations in your setup. eg: your equipment not being calibrated correctly. Ask your supervisor.

    You asked about this years ago.
    http://www.boards.ie/vbulletin/showthread.php?p=83273790


Advertisement