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Calibration curves

okman · 2016-04-28 12:43:01

Hi All, I recently did some experiments where i had to use a p-nitrophenol to create a calibration curve in order to calculate concentrations of p-nitrophenol in an assay. Now I realise these calibration curves are likely a little out, and i am potentially over estimating the concentrations of p-nitrophenol in my assay. Is…

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28-04-2016 1:43pm

#1

okman

Registered Users Posts: 17 ✭

Join Date: January 2008

Posts: 3

Hi All,

I recently did some experiments where i had to use a p-nitrophenol to create a calibration curve in order to calculate concentrations of p-nitrophenol in an assay.

Now I realise these calibration curves are likely a little out, and i am potentially over estimating the concentrations of p-nitrophenol in my assay.

Is it possible however, to use the extinction coefficients found in the literature for p-nitrophenl and calculate my concentrations using this data as opposed to that of my own calibration curves?

Cheers

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