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Stats for thesis

  • 28-03-2014 9:23pm
    #1
    supraspinaswim
    Registered Users, Registered Users 2 Posts: 80 ✭✭


    Not sure if anyone can help me here but Im doing a thesis on iNOS - inducible nitric oxide synthase induction in the CNS in an animal model of inflammatory bowel disease.

    Ive completed all the tissue prep, stained with antibodies, imaged it and counted it all. Ive been looking at inos expression and inos plus iba-1 expression - the activated microglial marker.

    The problem i have is trying to run stats on inos in the cns - ie should i get a total of inos and then the % overlap with iba-1 and run stats on it or is there anyother way to show that there is significant inos-iba-1 overlap ?

    The hypothesis is that microglia are the majority responsible for inos expression following the animal exposure to the IBD model. We looked at inos in the cns in control, acute and recovery stages, where acute and recovery got either 2 days recovery from the dss model or 5 days recovery.

    Any help at all would be appreciated !:eek:


Comments

  • #2
    Black Swan
    Moderators, Category Moderators, Science, Health & Environment Moderators, Society & Culture Moderators Posts: 47,532 CMod ✭✭✭✭Black Swan


    MOD: We will allow this enquiry into methods used to do this research. So if someone can help, please do.


  • #3
    mistress_gi
    Registered Users, Registered Users 2 Posts: 964 ✭✭✭mistress_gi


    I know I'm a bit late replying but here is my two cents anyway.
    Why not try a student T-test, this compares the mean of one population vs another being that your H0: the means are equal and H1: the mean of the villi is higher than the other
    I usually use graphpad for graphing and analysing all my results.
    Good luck!


  • #4
    supraspinaswim
    Registered Users, Registered Users 2 Posts: 80 ✭✭supraspinaswim


    Managed to figure out graphpad and the problem on the bus. ANOVA-one way and newman keuls, seems gp likes to make it awkward for the stats software rookies

    Thanks anyway !


  • #5
    djpbarry
    Registered Users, Registered Users 2 Posts: 13,104 ✭✭✭✭djpbarry


    supraspinaswim wrote: »
    Not sure if anyone can help me here but Im doing a thesis on iNOS - inducible nitric oxide synthase induction in the CNS in an animal model of inflammatory bowel disease.

    Ive completed all the tissue prep, stained with antibodies, imaged it and counted it all. Ive been looking at inos expression and inos plus iba-1 expression - the activated microglial marker.
    Can you give a bit more detail here? What exactly have you imaged and counted?


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