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Concentrate up an Extract from Biochar in DMSO

  • 16-06-2011 10:29am
    #1
    YFlyer
    Registered Users, Registered Users 2 Posts: 9,717 ✭✭✭


    Hi there

    Yesterday I extracted some organic material from different biochar samples (brown seaweed, miscanthus, poultry litter) using pure DMSO.

    I then attempted to concentrated up the supernatant using a rotary evaporator, but no success due to the high boiling point of DMSO.

    Would anybody know any other method for removing the DMSO and leaving behind the organic material?

    Thanks


Comments

  • #2
    AaronEnnis
    Registered Users, Registered Users 2 Posts: 151 ✭✭AaronEnnis


    You'll probably need a vacuum manifold as opposed to a standard rotevap. It'll take forever at atmospheric pressure. You may lose a good bit of product to DMSO as well.

    What do you need the extracted product for? Some assays can be run with the compound still in DMSO.


  • #3
    ianobrien
    Registered Users, Registered Users 2 Posts: 2,755 ✭✭✭ianobrien


    Have you tried SPE for concentration?

    Have a look at LLE for concentration/extraction?


  • #4
    Darando
    Registered Users, Registered Users 2 Posts: 732 ✭✭✭Darando


    I'm going to guess that the rotavap you are using has a water aspirator attached for vacuum?? If so then you will not remove DMSO with it.

    If you can get your hands on a small oil pump (Leybold/Edwards) that will do the job instead of the water aspirator. Did it many times before for high boiling solvents.


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