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Solubilise medium chain fatty acid powder :(

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  • 22-10-2010 9:54am
    #1
    me12345
    Closed Accounts Posts: 2 ✭


    Hey... im trying to solubilise a hydrophobic powder and not having much luck :( Ive tried mixing it with Ethanol or isopropanol but i dont think it is dissolving as the liquid is cloudy and one i dilute it in water...... it comes back out of solution :mad: I also tried mixing a bit of 1M HCl with the water and that worked a wee bit but not as much as id hoped ......

    I need to use the mixture to do in vitro bacterial inactivation tests so the medium used to dissolve it cannot be lethal to my bugs :). Would really appreciate any suggestions........ :confused::confused::confused::confused::confused::confused:


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    #2
    Gaspode
    Moderators, Category Moderators, Music Moderators, Regional East Moderators, Regional Midlands Moderators, Regional Midwest Moderators, Regional Abroad Moderators, Regional North Mods, Regional West Moderators, Regional South East Moderators, Regional North East Moderators, Regional North West Moderators, Regional South Moderators Posts: 8,008 CMod ✭✭✭✭Gaspode


    Have you tried a surfactant like tween? At low concentrations (around 0.5-1% v/v) it is not toxic to most bacteria.


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    #3
    mloc
    Registered Users Posts: 2,567 ✭✭✭mloc


    Have you tried DMSO? What concentration are you trying to dissolve it at?


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    #4
    CramCycle
    Moderators, Sports Moderators Posts: 24,495 Mod ✭✭✭✭CramCycle


    mloc wrote: »
    Have you tried DMSO? What concentration are you trying to dissolve it at?

    DMSO is known to induce mutations. I know those of us that do cell culture use it all the time for freezing down but this is more out of necessity than actual want. I think the Tween is your best bet as mentioned by Gaspode.


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    #5
    avalon68
    Registered Users Posts: 689 ✭✭✭avalon68


    Could you try sonication? - Some people in my lab use it for getting difficult compounds into solution.


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