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Hey guys, what are the advantages of Fluorescence Spectroscopy over FT-IR?

  • 05-02-2009 1:55am
    #1
    [Deleted User]
    Posts: 8,647 ✭✭✭


    Hey guys, been working on my final year project.I am absolutely wrecked.I can't find any info.My brain is absolutely fried.Any help from chem heads would be much apreciated?


Comments

  • #2
    sunnyjim
    Closed Accounts Posts: 1,302 ✭✭✭sunnyjim


    Benefits of Fluoresence Spec over FT-IR? Well you could thrown the LS out the window intoa skip and no one would care. I hate those pieces of ****.

    On that note, I was coming on here to ask some deets on where to find info on LS?


  • #3
    [Deleted User]
    Posts: 8,647 ✭✭✭ [Deleted User]


    sunnyjim wrote: »
    Benefits of Fluoresence Spec over FT-IR? Well you could thrown the LS out the window intoa skip and no one would care. I hate those pieces of ****.

    On that note, I was coming on here to ask some deets on where to find info on LS?

    PerkinElmer?


  • #4
    Staph
    Registered Users, Registered Users 2 Posts: 472 ✭✭Staph


    Advantages of fluorescence spec:

    Very sensitive compared to UV-vis spec
    Can be used for quantitation of fluorescent species
    Easy and quick to perform analysis

    Disadvantages:

    Not useful for identification (unlike IR)
    Not all compounds fluoresce
    Contamination can quench the fluorescence and hence give false/no results

    Advantages of FT-IR

    Can be used for identification of compounds (as long as you can interpret spectra)
    Easy sample preparation
    Qualitative
    Easy and quick to perform analysis

    Disadvantages:
    Not really quantitative


  • #5
    YFlyer
    Registered Users, Registered Users 2 Posts: 9,717 ✭✭✭YFlyer


    Staph wrote: »
    Advantages of fluorescence spec:

    Advantages of FT-IR

    Can be used for identification of compounds (as long as you can interpret spectra)

    Are you sure that should be functional groups


  • #6
    Staph
    Registered Users, Registered Users 2 Posts: 472 ✭✭Staph


    The spectra produced by FTIR enable one to ID the compound tested, by interpreting the distinct bands produced. Also where available, spectra can be compared to a reference library and the identity given.


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  • #7
    DemocAnarchis
    Registered Users, Registered Users 2 Posts: 404 ✭✭DemocAnarchis


    YFlyer wrote: »
    Are you sure that should be functional groups

    They can be used for identification of a known compound in conjunction with a library or standard as Staph said. They cannot be used to identify an unknown on their own without nmr/ms etc as complimentary techniques. FT-IR can identify functional groups, but can also differenciate between functional groups depending on adjacent groups, eg between the carbonyl of a carboxylic acid and aldehyde. They can also be used to identify aromatic substitution patterns and various stretching and bending vibrations.


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