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DNA stability

  • 20-10-2007 5:37pm
    #1
    2Scoops
    Registered Users, Registered Users 2 Posts: 1,845 ✭✭✭


    Let's say you were isolating DNA and the last step is reconstituting in TE at 65C for 1 hour. Let's also say you kept it at 65C overnight instead. :o What's going to happen to the DNA in terms of fragment length etc.? Still viable?


Comments

  • #2
    DrIndy
    Registered Users, Registered Users 2 Posts: 3,461 ✭✭✭DrIndy


    Did we do an oopsy? ;)

    I'm sure a few of the biochemistry/genetics minded heads will be along soon to give you a hand with this.....


  • #3
    vodochilie
    Closed Accounts Posts: 24 ✭vodochilie


    i pressume it's all denatured


  • #4
    2Scoops
    Registered Users, Registered Users 2 Posts: 1,845 ✭✭✭2Scoops


    The strands anneal again at lower temps.

    Anyway, figured it out myself the hard way - the fragment lengths get marginally shorter.


  • #5
    Tree
    Moderators, Science, Health & Environment Moderators Posts: 4,772 Mod ✭✭✭✭Tree


    but not significantly so?


  • #6
    2Scoops
    Registered Users, Registered Users 2 Posts: 1,845 ✭✭✭2Scoops


    Tree wrote: »
    but not significantly so?

    Statistically significant? I imagine with a large enough sample, yes.

    Meaningfully? Depends on what you want the DNA for...


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