JF Bio practicals?

paperclip

Tomorrow.

So...

any tips?

King.Penguin

Read over your lab manual and make sure all the questions are answered.

Pet

If you're in Nat Sciences, make sure you know your plant biochemistry stuff. I remember ****loads of that coming up.

paperclip

Do you remember any specific types of questions? I'm doing pharmacy, btw.

Pet

Hmm. They tend to be very specific--so know the theory behind your experiments well. Methods aren't so important like. I think pharmacy only do Michaelmas term of TR071 biology labs, and I can't remember many of those, aside from the SDS-PAGE one..

paperclip

that's right- we only did the SDS-PAGE one. Our biology course is truncated, since we do physiology as well and that went on for much longer. What sort of SDS-PAGE questions come up?

Pet

The specifics. pHs, molecular weights; what buffers and stains were used (think you used Coomassie blue, but can't remember); make sure you can read a picture of a finished one.

If you name some other experiments I might be able to throw something of use your way, because the only other experiments I can remember from MT were the mouse intestine one (be able to label the diagrams), and the pipetting one (be good at speedy serial-dilution calculations).

The questions go by fast--so you have to be quick off the mark; I think you get about 90 seconds before you have to switch places.

Pet

Pet wrote:

The questions go by fast--so you have to be quick off the mark; I think you get about 90 seconds before you have to switch places.

Musical chairs in an exam?

Pet

Yup, but with a bell instead of fun music, and the loser gets no marks and is forever shunned by all of the science faculty.

Kurtosis

Pet wrote:

Yup, but with a bell instead of fun music, and the loser gets no marks and is forever shunned by all of the science faculty.

I wanna play!

paperclip

A bell? Crap, being deaf will be a disadvantage here then.

ok, use & care of microscopes, plasmolysis, examination of plant cell protoplasts, cell staining, fixation & sectioning, histology staining, affinity chromatography....

thanks for all your help, btw!

Pet

I wouldn't think so - you'll see everyone moving, aside from those setups where you're gawking into a microscope (and not having a clue wtf you're looking at--but maybe that was just my shoddy attendance for microbiology labs..).

Edit: gawking into a microphone? Yes, I'm great at microbiology, me.

paperclip

what will be throuuugh the microscope?

John

Narnia. Or more likely they'll have prepared slides of typical bacteria, animal or plant cells and they'll ask you to identify them or say what the stain is/what the stain is staining.

Kurtosis

Dr Rochford said in his talk about this on Wednesday morning that in the case of any questions which involve looking at a specimen under the microscope, a colour photograph would accompany it, for anyone who didn't have their glasses with them, eye infection etc. He didn't give any clues as to what we'd be looking at though!

Hope it goes well for ya!

balzarywex

come on anatomy of the earthworm!:D

John

Ah JF biology, brings me back *wipes tear from eye*

paperclip

Thank you, these are fantastic, wonderful helpful pointers, people! Good luck on yours, penguin- put that E.Coli in its place, I tell you!

balzarywex

easiest exam ever! gotta love the blank questions... 90 seconds of just sitting there!:D