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16S rRNA

  • 03-03-2007 10:33pm
    #1
    smoke
    Closed Accounts Posts: 184 ✭✭


    Hi, just wondering about the length (bp) of 16s rRNA. I presume that since it is highly conserved in bacteria that there is a general length. Any help much appreciated.


Comments

  • #2
    Capt'n Midnight
    Moderators, Recreation & Hobbies Moderators, Science, Health & Environment Moderators, Technology & Internet Moderators Posts: 93,552 Mod ✭✭✭✭Capt'n Midnight


    Google for 16S rRNA kilodalton or 16S rRNA "base pairs long"


  • #3
    Tree
    Moderators, Science, Health & Environment Moderators Posts: 4,754 Mod ✭✭✭✭Tree


    16S is just the sediment rate. doesn't refer to a specific piece of
    RNA afaik


  • #4
    5c0ut3r
    Closed Accounts Posts: 3 ✭5c0ut3r


    16s rRNA does refer to a piece of RNA, specifically that from the ribosome.
    The lengths can vary depending on the group of organisms you are looking at (but usually not by much), so the estimate from this paper examining Bacillus 16s rRNA at 1,554 bp is probably not far off the mark. When you go across the domains of life however this doesn't hold all that well.
    For more info, check out wikipedia on the topic. But to see examples of sequences from different organisms go to the European Ribosomal RNA database


  • #5
    smoke
    Closed Accounts Posts: 184 ✭✭smoke


    thanks for the replies I can see that all of you but effort into your replies (i know the question was vague). I got the answer off a friend in the end.

    If you're interested...
    The PCR amplifies the RNA gene (DNA). I amplified a particuler stretch 200 bp in length due to the primers I used.

    Thanks again.


  • #6
    Marksie
    Closed Accounts Posts: 6,395 ✭✭✭Marksie


    smoke wrote:
    thanks for the replies I can see that all of you but effort into your replies (i know the question was vague). I got the answer off a friend in the end.

    If you're interested...
    The PCR amplifies the RNA gene (DNA). I amplified a particuler stretch 200 bp in length due to the primers I used.

    Thanks again.

    which wasn't the question you asked in the end....

    Is these for speciation by chance?


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  • #7
    r3nu4l
    Registered Users, Registered Users 2 Posts: 17,399 ✭✭✭✭r3nu4l


    5c0ut3r wrote:
    The lengths can vary depending on the group of organisms you are looking at (but usually not by much), so the estimate from this paper examining Bacillus 16s rRNA at 1,554 bp is probably not far off the mark.

    Exactly,

    Take this result from an entrez nucleotide search.

    16S rRNA from Streptococcus is 1557 bp. There are usually a good few differences but the general length is usually around 155X in bacteria.


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