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How genes from one organisms are

  • 16-08-2014 12:12pm
    #1
    carlowplayer
    Registered Users Posts: 519 ✭✭✭


    How does one transfer specific genes in say a plant into the genome of a bacteria step by step?


Comments

  • #2
    Furez
    Registered Users, Registered Users 2 Posts: 128 ✭✭Furez


    http://en.wikipedia.org/wiki/Transgenesis


  • #3
    carlowplayer
    Registered Users Posts: 519 ✭✭✭carlowplayer


    Furez wrote: »
    http://en.wikipedia.org/wiki/Transgenesis

    Thanks but that doesn't fully answer my question I mean how do you identify and isolate a desired gene(s) in a plant then amplyfy it and so on till you can transplant it into a bacterium. I ask because this has already been done with GM bacteria that can produce sugar, spider silk and rubber.


  • #4
    Furez
    Registered Users, Registered Users 2 Posts: 128 ✭✭Furez


    Yeah, that was a lazy answer. Google Isolation, Amplification and Transfer of genes. That is a lazy answer too.
    Maybe someone else knows the direct answer to your question. I think its amazing what can be done with microbes.

    Biotech


  • #5
    Capt'n Midnight
    Moderators, Recreation & Hobbies Moderators, Science, Health & Environment Moderators, Technology & Internet Moderators Posts: 92,450 Mod ✭✭✭✭Capt'n Midnight


    you can chop the donors DNA into random fragments and then incorporate them into the hosts randomly and hope for the best,

    then you test for the desired product - this is a lot easier if you something needed for growth



    next step up would be to use a plasmid that already has a testable gene, like production of an essential amino acid that you can withhold, only the organisms that receive the plasmid can grow , and you'd hope that the plasmid would carry the right DNA



    now we know the sequence of plasmids and restrictive enzymes can cut them at specific sequences leaving "sticky" ends and we know the alphabet of DNA even if we don't understand all of the language. so for simple proteins we can "print" the DNA sequence for and include some sticky ends to make it easy to incorporate into the plasmids.


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