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Common DNA in fruit or vegetable sequences

  • 05-12-2013 12:14PM
    #1
    CramCycle
    Moderators, Sports Moderators Posts: 26,373 Mod ✭✭✭✭


    I have identified primers for individual species of fruits but cannot find any papers which would have a general fruit or vegetable conserved sequence.
    Are there any out there or is it just a case of just picking out a known sequence for one of the fruits and see is it picked up by a huge nember of other species of fruit/veg when I run it through BLAST?


Comments

  • #2
    josey_whale
    Registered Users, Registered Users 2 Posts: 284 ✭✭josey_whale


    I've no experience of plant genetics - but, I think you should have a look some plant house keeping genes such as ribosomal RNA. I would imagine that you would be able to find universal primers for plant rRNA, intergenic spacers, ATP1, NAD or the genes involved in photosynthesis.

    Have a look at this paper They describe primers for ATP1

    ATP1_F TGAAYGAGATTYAAGYTGGGGAAATGGT
    ATP1_R CCCTCTTCCATCAATARRTACTCCCA


    I've BLASTed the forward primer and it is 100% homologous to everything it returned. There are a few degenerate bases in there, suggesting they could be what you are looking for.


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