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colorimetric assay

okman · 2013-02-18 13:03:06

Hey guys, Just wondering does anyone know how to use an extinction coefficient to calculate rate of hydrolysis of an enzyme. Simply put. For the experiment we incubate enzyme with substrate for known time, and read the absorbency of the incubation solution colorimetrically after 15mins. Then we using a calibration curve…

18-02-2013 1:03pm

#1

okman

Registered Users, Registered Users 2 Posts: 17 ✭

Join Date: January 2008

Posts: 3

Hey guys,

Just wondering does anyone know how to use an extinction coefficient to calculate rate of hydrolysis of an enzyme.

Simply put. For the experiment we incubate enzyme with substrate for known time, and read the absorbency of the incubation solution colorimetrically after 15mins. Then we using a calibration curve equation which has been determined for the product we can calculate the rate of hydrolysis. However for the enzyme I am looking at at the moment, I cannot create a calibration curve for the product. So in the literature they use the extinction coefficient apparently??

Anyone have any idea how this works??

Cheers

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