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Help - Gas Chromatography

  • 27-10-2009 4:22pm
    #1
    judgefudge
    Registered Users, Registered Users 2 Posts: 915 ✭✭✭


    Hi,

    I'm doing a write up for an experiment on cell immobilisation in ethanol fermentation. Just wondered can anyone help me with calculating the percentage ethanol from Gas Chromatography results. I've completely forgotten and I can't seem to find what I'm looking for anywhere online. The results we've got are in the form of Area Count, e.g. 2506613

    Appreciate it,
    E.


Comments

  • #2
    homah_7ft
    Registered Users, Registered Users 2 Posts: 6,126 ✭✭✭homah_7ft


    Might be missing something but have you run a standard injection?


  • #3
    YFlyer
    Registered Users, Registered Users 2 Posts: 9,717 ✭✭✭YFlyer


    Do you have the spreadsheet of all the peaks that came off the GC column and their relative areas? If yes, add up all the areas to get the total area. Then you can get the % of ethanol by (area of ethanol/total area)*100.

    If you want the actual concentration of ethanol. You may need to use an internal standard of known concentration. The area of the internal concentration that came off the GC column will be related to its concentration. The ethanol concentration will thereby be calculated by this same ratio.


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